There were no adverse events or serious adverse events related to the cell therapy. There was no evidence of additional spinal cord damage, mass lesion, or syrinx formation. We conclude that it is feasible to identify eligible candidates, appropriately obtain informed consent, perform a peripheral nerve harvest to obtain SCs within 5—30 days of injury, and perform an intra-spinal transplantation of highly purified autologous SCs within 4—7 weeks of injury.
The views expressed here are those of the authors and are not necessarily those of Journal of Neurotrauma, Mary Ann Liebert, Inc. No endorsement of any entity or technology is implied.
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Archives of Neuroscience | Polymeric Scaffolds in Neural Tissue Engineering: A Review
Top 10 Pharma Companies of Top 25 Biotech Companies of Top 10 Asia Biopharma Clusters Top 10 European Biopharma Clusters. Top 10 U. Major neurites were defined as those with the longest length. Recombinant lentiviruses were derived by the combined transfection of different plasmids as described Ricci-Vitiani et al. The protocol of lentiviral infection was then repeated three times in order to obtain different transduced-NPC clones.
The primer sequence information can be found in supplementary material Table S1. Western blot analysis was generally performed as previously described Merlo et al. Details on antibodies can be supplied on request. Electrical activity of the cell soma was recorded in the whole-cell configuration of the patch-clamp method Hamill et al. The signals were filtered at KHz and digitized at KHz. After the establishment of a gigaseal, the pipette resistance and capacitance were compensated electronically and the cells were accepted for study only if these parameters remained stable Zona et al.
Details of solution composition can be supplied on request. Anesthetized animals were subjected to a T7-T8 laminectomy. The NPCs or vehicle medium were slowly injected mm rostrally into the lesion at 0. Gait abnormalities in mice with contusion injury of the spinal cord were assessed weekly by footprint analysis using the CatWalk system Noldus, Wageningen, The Netherlands Hamers et al.
Briefly, the animals traverse a walkway in a dark room with a glass floor through which light is beamed from the long edge. Light is reflected completely internally. Only when the paw touches the floor light is the light deflected and exits the glass, so that only the contact area is visible.
The intensity of the signal depends on the pressure exerted, so that the spot will appear brighter when more weight is put on the paw. Animals crossing the walkway are videotaped using a computer-assisted setup, and digitized data are thresholded in order to extract the paw-floor contact areas and remove background.
At least three runs per animal were performed in each session. Labels were then assigned to the prints left and right, fore and hind paws and several parameters were measured by the Catwalk software, including: i base of support distance between the central pads of the hindfeet ; ii intensity mean brightness, in arbitrary units, of all pixels of the print at max contact; this is a measure of weight support of the different paws ; iii max area maximum area of a paw, in pixels, that comes into contact with the glass plate ; vi print area surface area, in pixels, of the complete print ; v print length length of the complete print ; vi print width width of the complete paw print ; vii stride length distance between two consecutive prints on each side ; and viii angle of rotation angle formed by the intersection of lines from the left and right prints results not shown.
Each mouse ran across the CatWalk several times before surgery to establish baseline locomotor parameters. To quantify the differentiation pattern of transplanted cells, we used confocal microscopy to count the number of EGFP-positive cells that were double-labeled with a different neuronal marker. We then counted the number of EGFP-positive cells that were double-labeled with the neuronal marker in three random fields per section. On average, cells were counted per field. This Issue. Download PDF.
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The authors talk about their work in and out of the lab, the journeys that led them to where they are now and the scientists who inspired them along the way. Where will your research take you? Here she studies the cause of chromosome mis-segregation defects in Xenopus hybrids. We caught up with Maiko to discuss her research, science communication, ballet, preprints and more.
The meeting will take place May , and further information is available here. A new poster from the Robinson lab summarises what is known about the five adaptor protein complexes and discuss how this helps to explain the clinical features of different genetic disorders.
Streamlining the publishing process, Review Commons enables high-quality peer review to take place before journal submission. Galko Development. McNally Development. Skip to main content. Research Article. Cristiana Mollinari. Materials and Methods Dissection and culturing Procedures involving animals and their care were conducted in strict accordance with the Policy on Ethics approved by the Society for Neuroscience, and with the European Communities Council Directive for Experimental Procedures. Immunofluorescence and confocal microscopy Immunofluorescence was performed as previously described Mollinari et al.
Western blotting Western blot analysis was generally performed as previously described Merlo et al.
Electrophysiology Electrical activity of the cell soma was recorded in the whole-cell configuration of the patch-clamp method Hamill et al. Behavioral assessment Gait abnormalities in mice with contusion injury of the spinal cord were assessed weekly by footprint analysis using the CatWalk system Noldus, Wageningen, The Netherlands Hamers et al.
Cancer Res. Barbeau, H.
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Initiation and modulation of the locomotor pattern in the adult chronic spinal cat by noradrenergic, serotonergic and dopaminergic drugs. Brain Res. Bixby, J. Purified N-cadherin is a potent substrate for the rapid induction of neurite outgrowth. Cell Biol. Boquet, I. Ciboulot regulates actin assembly during Drosophila brain metamorphosis.
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Long-term changes in the molecular composition of the glial scar and progressive increase of serotoninergic fibre sprouting after hemisection of the mouse spinal cord. Cao, Q. Stem cell repair of central nervous system injury. Carpintero, P. Expression of thymosin beta4 messenger RNA in normal and kainate-treated rat forebrain. Neuroscience 90 , Carunchio, I.
GAB A receptors present higher affinity and modified subunit composition in spinal motor neurons from a genetic model of amyotrophic lateral sclerosis. Castellani, V. Cis and trans interactions of L1 with neuropilin-1 control axonal responses to semaphorin 3A. EMBO J.
Safety of Autologous Human Schwann Cell Transplantation for Spinal Cord Injuries
Chen, H. Regulating actin-filament dynamics in vivo. Trends Biochem. Chen, J. Cell adhesion molecule l1-transfected embryonic stem cells with enhanced survival support regrowth of corticospinal tract axons in mice after spinal cord injury. Neurotrauma 22 , Adeno-associated virus-mediated L1 expression promotes functional recovery after spinal cord injury.
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